Four types of enzymes, working with surface cleaning detergents, achieve superior intraocular eye surgical instrument cleaning.
Eye surgical instrument cleaning detergents that do not contain four enzymes cannot effectively remove all forms of surgical bioburden. The four enzymes are: lipase enzymes to break down fat, amylase enzymes to break down starch, carbohydrase enzymes to break down high starches, and protease enzymes to break down blood and lipids emulsified with proteins.
Enzymes are not used up in a single cleaning reaction. They are available to for multiple cleaning reactions. The cleaning performance of enzymes continues but declines if they are overwhelmed by the volume of debris.
One type of enzyme is able to break down a specific type of bioburden, like a lock fits a key. The active site of an enzyme can only break down a specific type of debris. Specific types of enzymes are needed for effective cleaning the types of debris found on soiled intraocular eye surgical instruments, such as proteins (blood,) lipids, and starches. Proteins emulsified with lipids, found on phacoemulsification handpieces, present a significant cleaning challenge. Highly concentrated lipase with protease enzymes offer the highest probability of superior cleaning outcomes, for residue free intraocular eye surgical instruments.
Lipase Enzymes: break down fats to cleave fatty acid residue from the glycerol residue in a neutral fat or a phospholipid.
Amylase Enzymes: break down starches to catalyze the hydrolysis of starch to sugar to produce carbohydrate derivatives.
Carbohydrase Enzymes: break down starch to a lower levels to catalyze the hydrolysis of higher carbohydrates to lower forms.
Protease Enzymes: break down blood including the proteinases and peptidases, to catalyze the hydrolytic break down of proteins.
The proper use of cleaning times and temperatures render a higher probability of superior eye surgical instrument cleaning outcomes.
optimal enzyme temperatures for
The temperature for optimal enzyme cleaning performance peaks at 137 degrees Fahrenheit (58.33 degree Celsius). The cleaning activity of the enzyme at temperatures below and above this point is less but cleaning does occur. The cleaning activity of the enzyme does not stop at this peak temperature but is does lessen as the temperature increases or decreases. The cleaning temperatures do not impact the cleaning performance of detergents.
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